Feline Coronavirus (FCoV) belongs taxonomically to the family Coronaviridae, the genus of coronaviruses. It mainly infects young cats and is transmitted through the gastrointestinal tract, and young cats often show signs of enteritis when infected. The infection is mostly recessive in adult cats and can be fatal if malignant. The infection is mostly recessive in adult cats and can be fatal if malignant. Affected cats and healthy cats with the virus can excrete a large amount of the virus through the fecal route, and cats recovering from the disease can still carry the virus in their bodies but will not cause the disease. Cat coronavirus is weakly resistant to external physical and chemical factors and can be inactivated by most disinfectants.

The FCoV Test Kit ​is used to detect feline coronavirus antigens in the feces and vomit of cats.

Test principle of the FCoV -Ag Test Kit

The Cat Coronavirus Test Kit uses a rapid immunochromatographic assay technique to detect feline coronavirus antigens. Samples obtained from cat feces and vomit are added to the spiked wells and moved along the chromatographic membrane with a colloidal gold-labeled anti-FCoV monoclonal antibody. If the FCoV ag is present in the sample, it binds to the antibody on the detection line (T) and shows a burgundy color. If the FCoV antigen is absent in the sample, the feline coronavirus test cassette does not produce a color reaction.

The Feline Coronavirus Antigen Test Kit is able to detect Feline Coronavirus Antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of Feline Coronavirus in feces and vomit of cats. (FPV).

Sample Requirements

1. This kit is only suitable for detecting cat feces and vomit.
2. Collect fresh samples and immediately put them into the extraction buffer solution, which should be detected as soon as possible within 1 hour.
3. If samples are not timely detected, store them within 24 hours at 2-8℃ and store them within 1 month at -20℃.

How to Use Cat Coronavirus Antigen Test Kit?

1. Collect the eye secretions, nasal fluid and saliva samples with disposable swabs. After the samples were collected, they were stirred in the diluted solution and stood still for 1min. The cotton swabs were removed, the lid was tightened, and mixed upside down.
2. Tear open the aluminium foil bag of the test card, take out the test card, and put it on a flat and clean table.
3. Pipe 3 drops of sample solution (about 80μL), and add to the sample well of the test card.
4. Read test results within 10-15 min at room temperature, and those results beyond 20 min are invalid.

Note: The above reading time is conducted at the room temperature of 15-30℃. If the temperature is lower than 15℃, the reading time should be extended. If the temperature is higher than 30℃, the reading time should be shortened.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appears next to “C”.
Positive: In the test window, two distinct colored lines appear simultaneously next to “T” & “C”.
Invalid: no color line visible next to “C”.

NOTE: Invalid test results can be caused by various reasons and should be re-tested.

Interpretation of Test Results

1. The negative test results indicate the absence of feline coronavirus antigen in the samples. The infection cannot be ruled out if the corresponding acute symptoms occur.
2. The test results were positive, indicating samples containing feline coronavirus.
3. Multiple reasons may cause invalid results and should be re-tested.

Limitations of Feline Coronavirus Antigen Test Kit

• This kit can only detect the presence of canine distemper virus antigen in the samples examined.
• The test results are for clinical reference only and are not the basis for diagnosis.

Test principle of the Feline Coronavirus Antibody Test Kit

The Feline Coronavirus Antibody Test Kit detects antibodies to feline coronavirus using rapid immunochromatography. After whole cat blood, serum, or plasma is added to the sampling hole of the test tape, the sample is moved along the tomographic membrane with the colloidal gold labeled FCoV antigen. If the FCoV antibody is present in the sample, the detection line (T) will show red. No color will appear if the FCoV antibody is not present in the sample.

The Feline Coronavirus Antibody Test Kit is able to detect Feline Coronavirus antibody concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of feline coronavirus in feline whole blood, serum, or plasma. (FCoV).

Sample Requirements

• The feline Coronavirus Antibody Test Kit is only suitable for testing whole cat blood, serum, or plasma samples.
• The sample should be tested within 1 hour after collection.
• If timely testing is not possible and samples need to be properly stored, whole blood, serum, or plasma samples can be stored at 2-8℃ for 3 days, and serum or plasma samples can be stored at -20℃ for 1 month.

How to Use Canine Influenza Virus Antigen Test Kit?

1. Add the collected cat whole blood, serum, or plasma samples to the assay buffer, tighten the lid, and mix upside down.
2. Aspirate 3 drops (~80 μl) of diluted sample from the quantitative pipette into the spiked wells of the assay tape.
3. Wait for 10-15 min at room temperature to read the test results. After 20 min, the test results are invalid.

Interpretation of Results

Negative: Only the C line appears as a red color.
Positive: Both T and C lines appear purplish red.
Invalid: No color appears in the C line.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Feline Coronavirus Antibody Test Kit

• Do not use it if the product has expired or the foil bag is damaged.
• If the Feline Coronavirus Antibody Test Kit is removed from cryopreservation, it should be opened after returning to room temperature and used within 1 hour.
• Avoid contact with the sample wells and laminar strips on the test tape.