Heartfilariasis is a vector-borne disease of blood parasites caused by heartworm and Difilaria serpiginon in dogs. The main symptoms are circulatory disturbance, dyspnea and anemia.
This kit can be used to detect heartworm antigen in whole blood or serum of canine and feline for screening and auxiliary diagnosis of heartworm infection in canine and feline.

Test principle of the HW-Ag Test Kit

This product uses a rapid immunochromatographic technique for the detection of heartworm antigens in whole blood or serum of canine and feline. After the diluted sample was dropped into the sample well, if there was HW antigen in the sample, it would specifically bind to the recombinant protein labeled with colloidal gold to form a complex that moved along the chromatographic membrane and was captured by the HW antibody coated on the membrane, showing a wine red line on the detection line (T). If the HW antigen is not present in the sample, no visible line is formed at the T position. No matter the result is negative or positive, the C line should be colored, otherwise the result is invalid.

Sample Requirements

1. Please refer to the instructions for use
2. This kit is only suitable for testing feline whole blood or serum samples (hemolysis and hyperlipidemia samples cannot be used).
3. Collect fresh samples and immediately put into the extraction buffer solution, which should be detected as soon as possible within 1 hour.
4. If samples were not timely detected, store within 24 hours at 2-8°C and store within 1 month at -20°C.

Product Performance Indifelineors

1. Sensitivity ≥ 95%.
2. Specificity ≥ 97.5%.
3. Precision: no more than one false negative and false positive results within the batch; the test lines & control line (cards) between three lots shall be uniform, and no more than one time of color difference between lots.
4. Stability: valid for 24 month if unopened & store 2-30°C.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appear next to “C”.
Positive: In the test window, two distinct colored lines appear next to “T” & “C” simultaneously.
Invalid: No color line visible next to “C”.

NOTE: Invalid test results can be caused by various reasons and should be re-tested.