Toxoplasma Antigen Test Kit (TOXO-Ag Test Kit)

Intended Use of the Toxoplasma Antigen Test Kit

Toxoplasma gondii belongs to the smallest and structurally simple group of parasites called protozoa. Cats and other felines are the final hosts of Toxoplasma gondii, which is parasitized in the epithelial cells of the small intestine of these animals, forming cysts that are excreted in feces and eaten by other mammals and birds to become infected and develop into encysts in the tissues of their bodies. Cysts and encysts are different developmental stages of Toxoplasma gondii. However, it can only reproduce asexually in animals other than the end host and cannot disseminate its offspring to the outside world. This kit is used to detect Toxoplasma gondii antigen in the feces and rectum of animals.

Test Principle of the TOXO-Ag Test Kit

Rapid immunochromatographic assay technique for the detection of Toxoplasma gondii antigens. A sample from the rectum or stool is added to the spiked wells and moved along the chromatographic membrane with a colloidal gold-labeled anti-TOXO monoclonal antibody. If the TOXO antigen is present in the sample, it binds to the antibody on the detection line and appears burgundy. No colour reaction is produced if the TOXO antigen is not present in the sample.

The test serves only as an aid to the diagnosis. For professional use only.

Sample Requirements

1. The Toxoplasma Antigen Test Kit is only suitable for testing pet fecal and rectal samples.
2. The sample for the test must be collected immediately, the sample should be put into the dilution solution immediately after sampling, and the diluted sample should be tested as soon as possible, within 1 hour.
3. Diluted samples can be stored at 2-8°C for 24 hours and can be stored at -20°C for one month.

How to Use Toxoplasma Antigen Test Kit

1. Use disposable  swabs to collect fecal and rectal samples, put the samples into diluent and stir, stand still for 1min, take out the cotton swabs, tighten the lid, and mix upside down.
2. Tear open the aluminium foil bag of the test card, take out the test card, and put it on a flat and clean table.
3. Pipe 3 drops of sample solution (about 80μL), and add to the sample well of the test card.
4. Read test results within 10-15 min at room temperature, and those results beyond 20 min are invalid.

Note: The above reading time is conducted at the room temperature of 15-30℃. If the temperature is lower than 15℃, the reading time should be extended. If the temperature is higher than 30℃, the reading time should be shortened.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appears next to “C”.
Positive: In the test window, two distinct colored lines appear simultaneously next to “T” & “C”.
Invalid: no color line visible next to “C”.

NOTE: Invalid test results can be caused by various reasons and should be re-tested.

Interpretation of Test Results

1. The negative test results indicate the absence of toxoplasma antigen in the samples. The infection cannot be ruled out if the corresponding acute symptoms occur.
2. The test results were positive, indicating samples containing toxoplasma antigen.
3. Multiple reasons may cause invalid results and should be re-tested.

Limitations of Toxoplasma Antigen Test Kit

• This kit can only detect the presence of toxoplasma antigen in the samples examined.
• The test results are for clinical reference only and are not the basis for diagnosis.