Feline Coronavirus Antigen Test Kit (FCoV-Ag)

Intended Use of the Feline Coronavirus Antigen Test Kit

Feline Coronavirus (FCoV) belongs taxonomically to the family Coronaviridae, the genus of coronaviruses. It mainly infects young cats and is transmitted through the gastrointestinal tract, and young cats often show signs of enteritis when infected. The infection is mostly recessive in adult cats and can be fatal if malignant. The infection is mostly recessive in adult cats and can be fatal if malignant. Affected cats and healthy cats with the virus can excrete a large amount of the virus through the fecal route, and cats recovering from the disease can still carry the virus in their bodies but will not cause the disease. Cat coronavirus is weakly resistant to external physical and chemical factors and can be inactivated by most disinfectants.

The FCoV Test Kit ​is used to detect feline coronavirus antigens in the feces and vomit of cats.

Test principle of the FCoV -Ag Test Kit

The Cat Coronavirus Test Kit uses a rapid immunochromatographic assay technique to detect feline coronavirus antigens. Samples obtained from cat feces and vomit are added to the spiked wells and moved along the chromatographic membrane with a colloidal gold-labeled anti-FCoV monoclonal antibody. If the FCoV ag is present in the sample, it binds to the antibody on the detection line (T) and shows a burgundy color. If the FCoV antigen is absent in the sample, the feline coronavirus test cassette does not produce a color reaction.

The Feline Coronavirus Antigen Test Kit is able to detect Feline Coronavirus Antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of Feline Coronavirus in feces and vomit of cats. (FPV).

Sample Requirements

1. This kit is only suitable for detecting cat feces and vomit.
2. Collect fresh samples and immediately put them into the extraction buffer solution, which should be detected as soon as possible within 1 hour.
3. If samples are not timely detected, store them within 24 hours at 2-8℃ and store them within 1 month at -20℃.

How to Use Cat Coronavirus Antigen Test Kit?

1. Collect the eye secretions, nasal fluid and saliva samples with disposable swabs. After the samples were collected, they were stirred in the diluted solution and stood still for 1min. The cotton swabs were removed, the lid was tightened, and mixed upside down.
2. Tear open the aluminium foil bag of the test card, take out the test card, and put it on a flat and clean table.
3. Pipe 3 drops of sample solution (about 80μL), and add to the sample well of the test card.
4. Read test results within 10-15 min at room temperature, and those results beyond 20 min are invalid.

Note: The above reading time is conducted at the room temperature of 15-30℃. If the temperature is lower than 15℃, the reading time should be extended. If the temperature is higher than 30℃, the reading time should be shortened.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appears next to “C”.
Positive: In the test window, two distinct colored lines appear simultaneously next to “T” & “C”.
Invalid: no color line visible next to “C”.

NOTE: Invalid test results can be caused by various reasons and should be re-tested.

Interpretation of Test Results

1. The negative test results indicate the absence of feline coronavirus antigen in the samples. The infection cannot be ruled out if the corresponding acute symptoms occur.
2. The test results were positive, indicating samples containing feline coronavirus.
3. Multiple reasons may cause invalid results and should be re-tested.

Limitations of Feline Coronavirus Antigen Test Kit

• This kit can only detect the presence of canine distemper virus antigen in the samples examined.
• The test results are for clinical reference only and are not the basis for diagnosis.