Canine Parvovirus Antigen Test Kit (CPV-Ag)

Intended Use of the Canine Parvovirus Antigen Test Kit

Canine parvovirus (CPV) belongs to the family Parvoviridae, genus Parvovirus. Canine parvovirus is highly resistant to commonly used disinfectants and can survive in the feces for months to years. Canine microviruses are highly contagious and can infect dogs of different ages, sexes and breeds, and are particularly likely to infect underage puppies. Canine microvirus infection in dogs has a rapid onset and high mortality rate. The clinical manifestations are vomiting and diarrhea. The Canine Parvovirus Antigen Test Kit detects canine microvirus antigens in dogs’ feces, rectum, and vomit.

Test Principle of the Canine Parvovirus Antigen Test Kit

Rapid immunochromatographic assay technique for the detection of canine cerebrospinal virus antigen. A rectal or fecal sample is obtained and added to the spiked wells and then moved along the chromatographic membrane with a colloidal gold-labeled anti-CPV monoclonal antibody. If the CPV antigen is present in the sample, it binds to the antibody on the test line and appears burgundy. If the CPV antigen is not present in the sample, no color reaction is produced.

The Canine Parvovirus Antigen Test Kit is able to detect Canine Parvovirus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of Canine Parvovirus in the canine eye and conjunctival secretions, nasal fluid, saliva, feces, rectum, and vomit. (CPV).

Sample Requirements

1. This kit is only suitable for testing canine fecal, rectal and vomit samples.
2. The test sample is collected fresh and put into dilution solution immediately, and the diluted sample should be tested as soon as possible within 1 hour.
3. If the sample cannot be tested in time, the diluted sample can be stored at 2-8℃ for 24 hours and -20℃ for 1 month.

How to Use CPV-Ag Test Kit

1. Disposable swabs were used to collect samples of canine feces, rectum, and vomit. After collection, the samples were put into the dilution solution and stirred, left for 1 min, removed the swabs, tightened the lid, and mixed upside down.
2. Tear open the aluminum foil bag of the test card, remove the test card and place it on a flat, clean counter top.
3. Pipette 3 drops (about 80 μl) of the diluted sample into the spiked well of the test card and wait for the test result.
4. Wait for 10-15 min at room temperature to read the test results, after 20 min the test results are invalid.

Note: The above reading time is conducted at a room temperature of 15-30℃. The reading time should be extended if the temperature is lower than 15℃. If the temperature is higher than 30℃, the reading time should be shortened.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appears next to “C”.
Positive: In the test window, two distinct colored lines appear simultaneously next to “T” & “C”.
Invalid: no color line visible next to “C”.

NOTE: Invalid test results can be caused by various reasons and should be re-tested.

Interpretation of Test Results

1. The negative test results indicate the absence of the canine parvovirus in the samples. The infection cannot be ruled out if the corresponding acute symptoms occur.
2. The test results were positive, indicating samples containing feline panleukopenia virus.
3. Multiple reasons may cause invalid results and should be re-tested.

Limitations of Canine Parvovirus Antigen Test Kit

• This kit can only detect the presence of canine parvovirus antigen in the samples examined.
• The test results are for clinical reference only and are not the basis for diagnosis.