Canine Rotavirus Antigen Test Kit (CRV-Ag)

Intended Use of the Canine Rotavirus Antigen Test Kit

Canine rotavirus is highly resistant to commonly used disinfectants and can survive for months in feces. The specific symptoms of infected canine rotavirus are highly dependent on their age.

This kit detects canine rotavirus antigen in dog feces or vomit and is used for screening and auxiliary diagnosis of canine rotavirus infection.

Test principle of the CRV-Ag Test Kit

This product uses a rapid immunochromatographic technique for the detection of canine rotavirus antigens in canine feces or vomit. If there is CRV antigen in the sample, it will specifically bind to the recombinant protein labeled with colloidal gold to form a complex that will move along the chromatographic membrane and be captured by the CRV antibody coated on the membrane, showing a wine red line on the detection line (T). If the CRV antigen is not present in the sample, no visible line is formed at the T position. No matter the result is negative or positive, the C line should be colored, otherwise the result is invalid.

Sample Requirements

1. Please refer to the instructions for use
2. This kit is only suitable for testing feline whole blood or serum samples (hemolysis and hyperlipidemia samples cannot be used).
3. Collect fresh samples and immediately put into the extraction buffer solution, which should be detected as soon as possible within 1 hour.
4. If samples were not timely detected, store within 24 hours at 2-8°C and store within 1 month at -20°C.

Product Performance Indifelineors

1. Sensitivity ≥ 95%.
2. Specificity ≥ 97.5%.
3. Precision: no more than one false negative and false positive results within the batch; the test lines & control line (cards) between three lots shall be uniform, and no more than one time of color difference between lots.
4. Stability: valid for 24 month if unopened & store 2-30°C.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appear next to “C”.
Positive: In the test window, two distinct colored lines appear next to “T” & “C” simultaneously.
Invalid: No color line visible next to “C”.

NOTE: Invalid test results can be caused by various reasons and should be re-tested.