Canine Coronavirus Antigen Test Kit (CCV-Ag)

Intended Use of the Canine Coronavirus Antigen Test Kit

Canine coronavirus (CCV) is a single-win RNA virus with six to seven polypeptides, four of which are glycopeptides and do not contain RNA polymerase or neuraminidase. Corona in dogs can occur with varying degrees of gastroenteritis, characterized by frequent vomiting, diarrhea, depression, and anorexia. The disease can occur throughout the year but is more prevalent in winter, and the sick dog is the leading infectious agent. The Canine Coronavirus Antigen Test Kit is used to detect canine coronavirus antigens in the feces and rectum of dogs.

Test Principle of the Canine Coronavirus Antigen Test Kit 

The Canine Coronavirus Antigen Test Kit uses a rapid immunochromatographic assay to detect canine coronavirus antigen.
A rectal or fecal sample is added to the spiked wells and then moved along the chromatographic membrane with a colloidal gold-labeled anti-CCV monoclonal antibody. If the CCV antigen is present in the sample, it binds to the antibody on the test line and appears red. No color reaction is produced if the CCV antigen is not present in the sample.

The Canine Coronavirus Antigen Test Kit is able to detect canine coronavirus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Usec

For rapid screening of Canine Coronavirus in the feces and rectum (CCV).

Sample Requirements

1. This kit is only suitable for the detection of ocular secretions, nasal fluids, and saliva samples.
2. Test samples Collect fresh samples and put them in the diluent immediately. The diluted samples should be tested as soon as possible within 1 hour.
3. If it cannot be detected in time, the diluted samples can be stored at 2-8°C for 24 hours and at -20°C for 1 month.

How to Use CCV-Ag Test Kit

1. Use disposable cotton swabs to collect dog fecal and rectal samples, put the samples into diluent and stir, stand still for 1min, take out the cotton swabs, tighten the lid, and mix upside down.
2. Tear open the aluminium foil bag of the test card, remove the test card and place it on a flat, clean countertop.
3. Pipette 3 drops (about 80 μl) of the diluted sample into the spiked well of the test card and wait for the test result.
4. Wait for 10-15 min at room temperature to read the test results. After 20 min the test results are invalid.

Note: The above reading time is conducted at a room temperature of 15-30℃. The reading time should be extended if the temperature is lower than 15℃. If the temperature is higher than 30℃, the reading time should be shortened.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appears next to “C”.
Positive: In the test window, two distinct colored lines appear simultaneously next to “T” & “C”.
Invalid: no color line visible next to “C”.

NOTE: Invalid test results can be caused by various reasons and should be re-tested.

Interpretation of Test Results

1. The negative test results indicate the absence of canine coronavirus in the samples. The infection cannot be ruled out if the corresponding acute symptoms occur.
2. The test results were positive, indicating samples containing canine coronavirus.
3. Multiple reasons may cause invalid results and should be re-tested.

Limitations of Canine Coronavirus Antigen Test Kit

• This kit can only detect the presence of canine coronavirus antigen in the samples examined.
• The test results are for clinical reference only and are not the basis for diagnosis.